It is clear from the analysis that the linear relationship is optimal for the detection of glutathione at 1,654 cm 1 for the silver nanoparticles IR-enhanced substrate, and therefore the intensity of the IR peak at 1,654 cm 1 is selected for the subsequent quantification of glutathione

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Reviewers, including the Indian Journal of Dermatology, Venereology and Leprology paper on this exact topic, note that how long brightening lasts after stopping hasn't been properly studied yet treat it as maintenance, not a one-time fix
10.1016/j.mimet.2011.06.010 J Microbiol Meth